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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Springer US

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DOI

10.1007/978-3-540-73147-4_11

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ISSN

1559-0291

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RegenerationBased Quantification of Coumarins Sc

Authors: Kiranmayee Rao Bhuvaneswari Chodisetti Suryakala Gandi Archana Giri P B Kavi Kishor
Publish Date: 2016/05/17
Volume: 180, Issue: 4, Pages: 766-779
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Abstract

Abutilon indicum exploited for its immense value has been propagated successfully through multiple shoot induction and somatic embryogenesis Direct regeneration 820 ± 083 shoots was achieved from nodal explants using 05 mg/l kinetin Kn in MS media The basal callus from nodal explants turned embryogenic on subsequent introduction of 02 mg/l TDZ into the Knsupplemented media giving rise to somatic embryos The embryogenic potential of calli expressed in terms of embryoforming capacity EFC increased from 815 EFC to 2095 EFC after plasmolysis The phytochemical analysis HPLC for the presence of scopoletin and scoparone has revealed a unique accumulation pattern with higher levels of scopoletin during the earlier stages and scoparone in the later stages of development The embryogenic calli contained the highest amount of coumarins 9920 ± 097 and 6103 ± 047 μg/gFW respectively followed by regenerated plant 943 ± 020 and 3636 ± 119 μg/gFW respectively obtained via somatic embryogenesis Rapid multiplication of A indicum equipped with two potent coumarins is important in order to meet the commercial demand for combat against dreadful diseases thereby providing a new platform for plantbased drugs and their manufacture on a commercial scale

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