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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Springer US

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DOI

10.1007/bf02977648

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ISSN

1559-0291

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Antibacterial and Antifungal Activity of Biopolyme

Authors: Anshu Sharma Prem Prakash Kamla Rawat Pratima R Solanki H B Bohidar
Publish Date: 2015/07/05
Volume: 177, Issue: 1, Pages: 267-277
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Abstract

In the present study the antimicrobial properties of modified biopolymers such as gelatin and agar have been investigated These biopolymers agar and gelatin are modified by dissolving in ionic liquid IL 1ethyl3methylimidazolium chloride C2mimCl and 1octyl3methyl imidazolium chloride C8mimCl solutions It was noticed that agarionogel AgIL gelatinionogel GBIL and gelatin organogel gelatinglycerol solution along with laponite nanoclay nanocomposite GANC formed are highly stable optically clear and transparent without any air bubbles The antimicrobial activity of these AgIL GBIL and GANC were analyzed for both gramnegative Escherichia coli Klebsiella pneumoniae and grampositive bacterial strains Staphylococcus aureus and Staphylococcus pyogenes and fungus A niger C albicans Antibacterial and antifungal activity studies were carried out at different dilutions such as 100 99 and 90  v/v It was found that AgIL GBIL and individual IL C8mimCl exhibited superior antimicrobial activities indicating that longer IL chain enhance the cell membrane permeability of S aureus S pyogenes and E coli cells However GANC nanocomposite and C2mimClbased composites does not exhibit any bacterial inhibition activity for all bacterial strainsAS acknowledges University Grants Commission Government of India for research fellowship KR acknowledges receipt of DST Inspire Faculty Award This work was supported by a grant received from Department of Science and Technology Government of India and University Grants Commission for startup grant

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