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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Springer-Verlag

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DOI

10.1002/asna.18961410403

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1559-0291

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Intrinsic Characteristics of CrSuperscript6+/Su

Authors: B Hemambika V Rajesh Kannan
Publish Date: 2012/02/28
Volume: 167, Issue: 6, Pages: 1653-1667
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Abstract

The Cr6+resistant plant growthpromoting bacteria was isolated from soil samples that were collected from an electroplating industry at Coimbatore India that had tolerated chromium concentrations up to 500 mg Cr6+/L in LuriaBertani medium Based on morphology physiology and biochemical characteristics the strain was identified as Bacillus sp following the Bergeys manual of determinative bacteriology Evaluation of plant growthpromoting parameters has revealed the intrinsic ability of the strain for the production of indole3acetic acid IAA siderophore and solubilization of insoluble phosphate Bacillus sp have utilized tryptophan as a precursor for their growth and produced IAA 122 μg/mL Bacillus sp also exhibited the production of siderophore that was tested qualitatively using Chrome Azurol S CAS assay solution and utilized the insoluble tricalcium phosphate as the sole source of phosphate exhibiting higher rate of phosphate solubilization after 72 h of incubation 145 μg/mL Extent of Cr6+ uptake and accumulation of Cr6+ in the cell wall of Bacillus sp was investigated using atomic absorption spectrophotometer and scanning electron microscopeenergy dispersive spectroscopy respectively The congenital capability of this Cr6+resistant plant growthpromoting Bacillus sp could be employed as bacterial inoculum for the improvement of phytoremediation in heavy metal contaminated soils

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