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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Springer-Verlag

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DOI

10.1007/bf00229525

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1559-0291

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Singlestep Purification and Immobilization of MBP

Authors: Mrudula Vasudevan Ushasree Paramasamy Gunasekaran Ashok Pandey
Publish Date: 2012/02/29
Volume: 167, Issue: 5, Pages: 981-990
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Abstract

Periplasmic phytase appA from E coli has been noticed as a superior feed and food additive owing to its high specific activity acidic pH optimum and resistance to gastric proteases E coli phytase was expressed as a fusion protein with maltosebinding protein affinitypurified to homogeneity and subsequently immobilized in one step using a costeffective matrix prepared from starch agar bead Immobilized enzyme revealed an activity optimum at pH 6 while that of free enzyme was observed at pH 4 Both the immobilized and free enzyme showed a temperature optimum at 60 °C Cleavage of 87 kDa fusion protein using factor Xa released 45 kDa appA Hydrolysis of soy milk using immobilized enzyme led to 10 increase in release of inorganic phosphate at 50 °C relative to free fusion protein This study suggests the usability of MBP as an immobilizing linker to other food enzymes for economical use in industry

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