Authors: Neera Karna Venkata Ramana Harsh Vardhan Batra
Publish Date: 2015/05/01
Volume: 176, Issue: 4, Pages: 1162-1173
Abstract
Cellulose producing bacteria were isolated from fruit samples and kombucha tea a fermented beverage using CuSO4 solution in modified Watanabe and Yamanaka medium to inhibit yeasts and molds Six bacterial strains showing cellulose production were isolated and identified by 16S rRNA gene sequencing as Gluconacetobacter xylinus strain DFBT Ga xylinus strain dfr1 Gluconobacter oxydans strain dfr2 G oxydans strain dfr3 Acetobacter orientalis strain dfr4 and Gluconacetobacter intermedius strain dfr5 All the celluloseproducing bacteria were checked for the cellulose yield A potent celluloseproducing bacterium ie Ga xylinus strain DFBT based on yield cellulose yield 56 g/L was selected for further studies Cellulose was also produced in non conventional media such as pineapple juice medium and hydrolysed corn starch medium A very high yield of 91 g/L cellulose was obtained in pineapple juice medium Fourier transform infrared spectrometer FTIR analysis of the bacterial cellulose showed the characteristic peaks Soft cellulose with a very high water holding capacity was produced using limited aeration Scanning electron microscopy SEM was used to analyze the surface characteristics of normal bacterial cellulose and soft cellulose The structural analysis of the polymer was performed using 13C solidstate nuclear magnetic resonance NMR More interfibrillar space was observed in the case of soft cellulose as compared to normal cellulose This soft cellulose can find potential applications in the food industry as it can be swallowed easily without chewing
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