Journal Title
Title of Journal: Appl Biochem Biotechnol
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Abbravation: Applied Biochemistry and Biotechnology
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Authors: Jesús Martín MorenoHernández Xitlalli Desideria HernándezMancillas Evelia Lorena Coss Navarrete Miguel Ángel MazorraManzano Idalia OsunaRuiz Víctor Alfonso RodríguezTirado Jesús Aarón SalazarLeyva
Publish Date: 2016/11/09
Volume: 182, Issue: 1, Pages: 181-196
Abstract
Plant proteases are capable of performing several functions in biological systems and their use is attractive for biotechnological process due to their interesting catalytic properties Bromelia pinguin aguama is a wild abundant natural resource in several regions of Central America and the Caribbean Islands but is underutilized Their fruits are rich in proteases with properties that are still unknown but they represent an attractive source of enzymes for biotechnological applications Thus the proteolytic activity in enzymatic crude extracts CEs from wild B pinguin fruits was partially characterized Enzymes in CEs showed high proteolytic activity at acid pH 20–40 and neutral alkaline pH 70–90 conditions indicating that different types of active proteases are present Proteolytic activity inhibition by the use of specific protease inhibitors indicated that aspartic cysteine and serine proteases are the main types of proteases present in CEs Activity at pH 30 was stable in a broad range of temperatures 25–50 °C and retained its activity in the presence of surfactants SDS Tween80 reducing agents DTT 2mercapoethanol and organic solvents methanol ethanol acetone 2propanol which suggests that B pinguin proteases are potential candidates for their application in brewing detergent and pharmaceutical industriesThe first author wants to thank the National Council of Science and Technology of Mexico CONACyT for the economic support during postgraduate studies HernándezMancillas and CossNavarrete wish to thank the “Programa Interinstitucional para el Fortalecimiento de la Investigación y el Posgrado del Pacífico DELFIN” for the received summer scholarship We thank Gerardo Reyna for the bibliography support during this research
Keywords:
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