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Title of Journal: Appl Biochem Biotechnol

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Abbravation: Applied Biochemistry and Biotechnology

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Springer US

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DOI

10.1002/lipi.19820840612

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1559-0291

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Improvement of RegioSpecific Production of Myrice

Authors: Nguyen Huy Thuan Ramesh Prasad Pandey Ta Thi Thu Thuy Je Won Park Jae Kyung Sohng
Publish Date: 2013/09/08
Volume: 171, Issue: 8, Pages: 1956-1967
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Abstract

Myricetin is an important flavonol whose medically important properties include activities as an antioxidant anticarcinogen and antimutagen The solubility stability and other biological properties of the compounds can be enhanced by conjugating aglycon with sugar moieties The type of sugar moiety also plays a significant role in the biological and physical properties of the natural product glycosides Reconstructed Escherichia coli containing thymidine diphosphateαlrhamnose sugar gene cassette and Arabidopsisderived glycosyltransferase were used for rhamnosylation of myricetin Myricetin 100 μM was exogenously supplemented to induced cultures of engineered E coli The formation of target product—myricetin3Oαlrhamnoside—was confirmed by chromatographic and NMR analyses The yield of product was improved by using various mutants and methylated cyclodextrin as a molecular carrier for myricetin in combination with E coli M3G3 The maximal yield of product is 556 μM 331fold higher than the control E coli MG3 and shows 556  bioconversion of substrate under optimized conditionsThis work was supported by grant from the NextGeneration BioGreen 21 Program SSAC grant PJ00948302 Rural Development Administration and supported by the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Education Science and Technology 20110031960 Republic of Korea grants from the National Foundation for Science and Technology Development—NAFOSTED Vietnam Grant no 10401201022

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