Authors: Nayeli ÁvilaCisneros Susana VelascoLozano Sergio HuertaOchoa Jesús CórdovaLópez Miquel Gimeno Ernesto FavelaTorres
Publish Date: 2014/08/23
Volume: 174, Issue: 5, Pages: 1859-1872
Abstract
A naturally immobilized biocatalyst with lipase activity was produced by Thermomyces lanuginosus on solidstate fermentation with perlite as inert support Maxima lipase activities 22 and 120 U/g of dry matter using pnitrophenyl octanoate and trioctanoine respectively as substrates were obtained after 72 h of solid culture remaining nearly constant up to 120 h Maxima lipase activity was found at 60 to 85 °C and pH 10 The biocatalyst was stable at 60 °C for at least 4 h of incubation and a pH from 7 to 10 Energy values of activation and deactivation of lipase were of 26 and 67 kJ/mol respectively The biocatalyst shows high selectivity for the release of the omega3 polyunsaturated fatty acids eicosapentaenoic EPA and docosahexaenoic acids DHA during the hydrolysis of sardine oil The EPA/DHA ratio 166 released by this biocatalyst was superior to that obtained with the commercial preparations of T lanuginosusThe authors acknowledge financial support Project 154004 from The National Council for Science and Technology CONACyT and Nayeli Ávila acknowledges the scholarship number 203453 The authors gratefully acknowledge Dr Gloria FernándezLorente for her support and technical assistance during the application of biocatalysts in the hydrolysis of sardine oil at the Research Institute of Food Science CIAL UAM Madrid Spain
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