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Title of Journal: J Microbiol

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Abbravation: Journal of Microbiology

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Publisher

The Microbiological Society of Korea

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DOI

10.1002/bit.260310910

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ISSN

1976-3794

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Genomewide enrichment screening reveals multiple

Authors: Byung Jo Yu Jung Ae Kim Hyun Mok Ju SooKyung Choi Seung Jin Hwang Sungyoo Park EuiJoong Kim JaeGu Pan
Publish Date: 2012/11/04
Volume: 50, Issue: 5, Pages: 785-791
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Abstract

Triclosan is a widely used biocide effective against different microorganisms At bactericidal concentrations triclosan appears to affect multiple targets while at bacteriostatic concentrations triclosan targets FabI The sitespecific antibioticlike modeofaction and a widespread use of triclosan in household products claimed to possibly induce crossresistance to other antibiotics Thus we set out to define more systematically the genes conferring resistance to triclosan A genomic library of Escherichia coli strain W3110 was constructed and enriched in a selective medium containing a lethal concentration of triclosan The genes enabling growth in the presence of triclosan were identified by using a DNA microarray and confirmed consequently by ASKA clones overexpressing the selected 62 candidate genes Among these fortyseven genes were further confirmed to enhance the resistance to triclosan these genes including the FabI target were involved in inner or outer membrane synthesis cellsurface material synthesis transcriptional activation sugar phosphotransferase PTS systems various transporter systems cell division and ATPase and reductase/dehydrogenase reactions In particular overexpression of pgsA rcsA or gapC conferred to E coli cells a similar level of triclosan resistance induced by fabI overexpression These results indicate that triclosan may have multiple targets other than wellknown FabI and that there are several undefined novel mechanisms for the resistance development to triclosan thus probably inducing cross antibiotic resistance

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