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Title of Journal: J Microbiol

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Abbravation: Journal of Microbiology

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Springer Netherlands

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DOI

10.1016/0001-6160(66)90165-9

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ISSN

1976-3794

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Surface display of the HPV L1 capsid protein by th

Authors: Dan Xu Xiaofeng Yang Depu Wang Jun Yu Yili Wang
Publish Date: 2014/01/04
Volume: 52, Issue: 1, Pages: 77-82
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Abstract

Autotransporters have become attractive tools for surface expression of foreign proteins in Gramnegative bacteria In this study the Shigella autotransporter IcsA has been exploited to express the human papillomavirus HPV type 16 L1 capsid protein in Shigella sonnei and Escherichia coli The L1 gene was fused inframe to replace the coding sequence of the IcsA passenger domain that is responsible for actinbased motility The resultant hybrid protein could be detected by an antiL1 antibody on the surface of S sonnei and E coli In E coli the protein was expressed on the entire surface of the bacterium In contrast the protein was detected mainly at one pole of the Shigella bacterium However the protein became evenly distributed on the surface of the Shigella bacterium when the icsP gene was removed Our study demonstrated the possibility of exploiting autotransporters for surface expression of large heterologous viral proteins which may be a useful strategy for vaccine development

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