Authors: JinKwang Kim MyungSuk Kang Sung Chul Park KyengMin Kim Kangduk Choi MinHo Yoon WanTaek Im
Publish Date: 2015/06/27
Volume: 53, Issue: 7, Pages: 435-441
Abstract
The Gramreactionnegative strictly aerobic nonmotile nonsporeforming and rodshaped bacterial strain designated BS11T was isolated from the compost and its taxonomic position was investigated by using a polyphasic approach Strain BS11T grew optimally at 30–37°C and at pH 70 in the absence of NaCl on nutrient agar Strain BS11T displayed βglucosidase activity that was responsible for its ability to transform ginsenoside Rb1 one of the dominant active components of ginseng to Rd On the basis of 16S rRNA gene sequence similarity strain BS11T was shown to belong to the family Sphingomonadaceae and was related to Sphingosinicella vermicomposti YC7378T 963 sequence similarity S xenopeptidilytica 3–2W4T 962 S microcystinivorans Y2T 961 and S soli KSL125T 959 The G+C content of the genomic DNA was 649 The major menaquinone was Q10 and the major fatty acids were summed feature 7 comprising C181 ω7c/ω9t/ω12t 406 C160 225 C171 ω6c 137 and C170 91 DNA and chemotaxonomic data supported the affiliation of strain BS11T to the genus Sphingosinicella Strain BS11T could be differentiated genotypically and phenotypically from the recognized species of the genus Sphingosinicella The novel isolate therefore represents a novel species for which the name Sphingosinicella ginsenosidimutans sp nov is proposed with the type strain BS11T =KACC 16619T =JCM 18201T
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