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Title of Journal: Conserv Genet

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Abbravation: Conservation Genetics

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Springer Netherlands

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DOI

10.1016/j.artres.2008.08.345

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ISSN

1572-9737

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Identification and characterization of ESTSSRs an

Authors: ZhengFeng Wang WanHui Ye HongLin Cao ZhongChao Li ShaoLin Peng
Publish Date: 2007/11/18
Volume: 9, Issue: 4, Pages: 1079-1081
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Abstract

We report on the identification and characterization of six ESTlinked simple sequence repeats ESTSSRs and chloroplast SSRs cpSSRs in endangered Cycas hainanensis The number of alleles ranged from two to eight for ESTSSRs two to three for cpSSRs Observed and expected heterozygosities ranged from 0042 to 0417 and 0042 to 0811 for ESTSSRs respectively Expected heterozygosities ranged from 0156 to 0457 for cpSSRs All these markers gave successful crossspecies amplification in C fairylakea These markers will allow analyses of the baseline genetic variability and population structure of C hainanensis to provide strategies for effective conservation and managementWe thank Peng Zeqiu and Jian shuguang for helping collect/identify samples This work was supported by National Basic Research Program of China 973 Program No 2007CB411600 the Knowledge Innovation Program of the Chinese Academy of Sciences Grant No KSCX2YWZ023 and Science Utility Project of Guangdong Province No 2005B60301001

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  7. Genetic diversity of an endangered plant, Cypripedium macranthos var. rebunense (Orchidaceae): background genetic research for future conservation
  8. Species identification and genetic structure of threatened seahorses in Gran Canaria Island (Spain) using mitochondrial and microsatellite markers
  9. Development and application of a molecular sexing protocol in the climate change-sensitive American pika
  10. Development and characterization of 18 microsatellite loci for the spotted salamander ( Ambystoma maculatum ) using paired-end Illumina shotgun sequencing
  11. Development of microsatellite markers for Adenophora palustris (Campanulaceae), a critically endangered wetland plant species in Japan
  12. The effect of reintroductions on the genetic variability in Eurasian lynx populations: the cases of Bohemian–Bavarian and Vosges–Palatinian populations
  13. Isolation and characterization of 16 microsatellite markers in Intsia palembanica , a high-value tropical hardwood species
  14. Development of 16 microsatellite loci for the endangered sandhill dunnart ( Sminthopsis psammophila )
  15. Fecal samples fail in PCR-based diagnosis of malaria parasite infection in birds
  16. Isolation and characterization of 12 microsatellite loci from the endemic Schizothorax chongi (Fang, 1936)
  17. The development of 24 polymorphic microsatellite loci for the endangered barred galaxias, Galaxias fuscus , through next generation DNA sequencing
  18. Isolation and characterization of novel microsatellite markers in Gekko gecko (Reptilia: Gekkonidae)
  19. Twenty-nine microsatellite markers for two Australian freshwater turtles, Elseya albagula and Emydura macquarii krefftii : development from 454-sequence data and utility in related taxa
  20. Isolation and characterization of microsatellite markers for Cichla monoculus (Agassiz, 1831), an important freshwater fish in the Amazon
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  22. Isolation and characterization of twelve novel polymorphic microsatellite loci in the Branchiostoma belcheri Gray (Amphioxus)
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  25. Development of nine polymorphic microsatellite loci in the squirrel glider ( Petaurus norfolcensis )
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  28. Natural, not urban, barriers define population structure for a coastal endemic butterfly
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  30. Applying QTL analysis to conservation genetics
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  43. A rapid method for species identification and interspecies introgression based on a PCR–RFLP: application to the closely related Neotropical toads Rhinella marina and R. schneideri (Anura: Bufonidae)
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  50. Isolation and characterization of eleven polymorphic microsatellite markers of sand lance ( Ammodytes personatus )
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