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Title of Journal: Conserv Genet

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Abbravation: Conservation Genetics

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Springer Netherlands

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DOI

10.1016/0029-5493(82)90142-x

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ISSN

1572-9737

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Development and characterization of microsatellite

Authors: Eddy José Francisco Oliveira José Eduardo Garcia José Mauricio Barbanti Duarte Eucleia Primo Betioli Contel
Publish Date: 2008/12/11
Volume: 10, Issue: 5, Pages: 1505-
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Abstract

Marsh deer Blastocerus dichotomus is one of the most exposed large mammals in South America To aid in the conservation management of the species nine polymorphic microsatellite loci were isolated and tested on up to 50 animals showing 3–12 alleles and expected heterozygosity values varying from 069 to 089 These markers should be of considerable utility in future population and ecological genetics studies of this species The marsh deer Blastocerus dichotomus is the biggest South American species of deer Originally distributed across a large part of South America stretching from the south bank of the Amazon river to northern Argentina significant wild populations are now restricted to the Pantanal swamplands that cover about 40 of southwest Brazil The marsh deer is listed as Vulnerable on the Red List of the IUCN Three populations of the species from three areas in the Paraná River basin between the states of São Paulo and Mato Grosso do Sul were recently studied by observing protein polymorphism at 17 loci Oliveira et al 2005 Now we are presenting data about isolation of microsatellite markers to improve the results regarding population structureThis work was supported by CESP Companhia Energética de São Paulo CNPq Conselho Nacional de Desenvolvimento Científico e Tecnológico and FAEPA Fundação de Apoio ao Ensino Pesquisa e Assistência do HCFMRP Thanks are due to CAPES Coordenação de Aperfeiçoamento de Pessoal de Nível Superior for fellowships granted to EJF Oliveira and JEGarcia We also thank Ângela Talarico for assistance with sequencing

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