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Title of Journal: Conserv Genet

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Abbravation: Conservation Genetics

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Publisher

Springer Netherlands

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DOI

10.1007/bf03055020

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ISSN

1572-9737

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Development and characterization of 15 microsatell

Authors: Marcela Corbo Guidugli Tatiana de Campos Adna Cristina Barbosa de Sousa Juliana Massimino Feres Alexandre Magno Sebbenn Moacyr Antonio Mestriner Eucleia Primo Betioli Contel Ana Lilia AlzateMarin
Publish Date: 2008/08/29
Volume: 10, Issue: 4, Pages: 1001-
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Abstract

From a genomic library enriched for GA/CA repeats 15 highly polymorphic microsatellite markers were developed for Cariniana estrellensis a tropical forest tree The microsatellite loci were screened in 49 mature trees found between Pardo river and MogiGuaçu river basins in the state of São Paulo Brazil A total of 140 alleles were detected with an average of 933 alleles per locus The expected heterozygosity ranged from 037 to 088 These loci showed a high probability of paternity exclusion Additionally 12 loci were effectively transferred to Cariniana legalis High levels of polymorphism make the present SSR markers useful for population genetic studiesThis work was financed by a grant from São Paulo State Government to Ana Lilia AlzateMarin Fundação de Amparo à Pesquisa do Estado de São Paulo FAPESP 03/041994 We also acknowledge the support of PróReitoria de Pesquisa and Fundação de Apoio ao Ensino Pesquisa e Assistência FAEPA of São Paulo University This study is part of the postgraduate work of first author Ana Lilia AlzateMarin was supported by Researcher assistantship from FAPESP Marcela Corbo Guidugli was supported by a doctoral fellowship of FAPESP 07/047874 The authors would like to thank to Dr Anete Pereira de Souza for scientific support and Ronai Ferreira Ramos for technical support in primers design

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