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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1007/s10357-009-1751-x

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1573-6776

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pEGFPT a novel Tvector for the direct unidirec

Authors: Baoli Wang Xiaoxia Li Fang Zheng Rui Liu Jinxing Quan Hui Liang Shanyi Guo Gang Guo Jingyu Zhang
Publish Date: 2006/11/22
Volume: 29, Issue: 2, Pages: 309-312
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Abstract

A novel Tvector designated as pEGFPT was constructed which could be used for direct and unidirectional cloning and analysis of promoters The method involves minimizing the nonrecombinant background of the Tvector when used to clone PCR products and maintaining only the forward orientation of the PCR products prior to transformation The usefulness of this vector is demonstrated in cloning and analyzing promoters

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  5. Biohydrogen production by Enterobacter cloacae and Citrobacter freundii in carrier induced granules
  6. High-yield production of short chain length poly(ε- l -lysine) consisting of 5–20 residues by Streptomyces aureofaciens , and its antimicrobial activity
  7. Isolation and characterization of a novel ε-caprolactam-degrading microbe, Acinetobacter calcoaceticus , from industrial wastewater by chemostat-enrichment
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  9. Enzymatic transformation of nitro-aromatic compounds by a flavin-free NADH azoreductase from Lysinibacillus sphaericus
  10. Expression of truncated tobacco osmotin in Escherichia coli : purification and antifungal activity
  11. Isolation and functional characterization of polyunsaturated fatty acid elongase (AsELOVL5) gene from black seabream ( Acanthopagrus schlegelii )
  12. In vitro generation of type-II pneumocytes can be initiated in human CD34 + stem cells
  13. Electrospun silk fibroin mat enhances tendon-bone healing in a rabbit extra-articular model
  14. Remediation of the effect of adding cyanides on an algal/bacterial treatment of a mixture of organic pollutants in a continuous photobioreactor
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