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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1007/978-1-4684-5227-3_1

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1573-6776

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Silk screen based dual spinfilter module for perf

Authors: Shweta Kamthan James Gomes Pradip K Roychoudhury
Publish Date: 2014/04/16
Volume: 36, Issue: 8, Pages: 1623-1630
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Abstract

Spinfilters have been primarily used for producing therapeutic proteins from mammalian cells However disposability and/or high filter clogging of the existing spinfilter systems affect the process economy and productivity Hence to address these drawbacks a reusable dual spinfilter module for perfusion culture of adherent and nonadherent mammalian cells was designed Two nonwoven Bombyx mori silk layers were used as filter screen the outer layer was conducive to cell attachment whilst the inner was nonconducive Adherent cells can be cultured either in suspended mode using its inner single module or as monolayer of cells using its dual concentric module We achieved 30  higher urokinase productivity as compared to the stainlesssteel spinfilter during perfusion experiments of adherent human kidney cells in suspended mode This was due to the hydrophobic and negativelycharged silk screen that allows clogfree perfusion culture for prolonged periods

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