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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1007/bf01841453

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1573-6776

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Isolation of sensitive nisinsensing GFPSubscript

Authors: Hanan T Abbas Hilmi Kaisa Hakkila Per E J Saris
Publish Date: 2008/09/18
Volume: 31, Issue: 1, Pages: 119-122
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Abstract

Fluorescenceactivated cell sorting FACS was used to isolate mutants of Lactococcus lactis LAC275 an indicator strain in GFPuv nisin bioassay It harbors the GFPuv encoding gene under the nisA promoter and the nisin signal transduction nisRK genes whereby nisin concentration can be correlated to GFPuv fluorescence The sorted L lactis cells which showed higher fluorescence intensities at low inducer concentration were analysed for higher responsiveness to low concentration of nisin Two strains showed lower detection limits 02 pg ml−1 for nisin than the parent strain 10 pg ml−1 This showed that mutants of LAC275 could successfully be isolated using FACS

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