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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1007/s11085-016-9615-2

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1573-6776

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Lentiviral vectorderived shRNAs confer enhanced s

Authors: Ke Zhang Yu Chen Ji’an Pan Tero Ahola Deyin Guo
Publish Date: 2008/12/31
Volume: 31, Issue: 4, Pages: 501-508
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Abstract

Semliki forest virus SFV is a pathogen causing lethal encephalitis in laboratory mice In this study we obtained three short hairpin RNAs shRNAs which could specifically target SFV sequence in GFP reporting systems and effectively suppress SFV replication in luciferasecontaining reporter virus system At a multiplicity of infection MOI of 0001 the luciferase reporter activity was reduced by 78–92 by shRNA expression plasmids and virus yields reduced 2 to 10fold at 20 h postinfection When lentiviral vectorderived shRNAs were employed the virus titers decreased 8 to 126fold at 24 h postinfection and 6 to 19fold at 48 h postinfection and the cell survival was prolonged These data formed the basis for further in vivo studies of RNA interference in mouse models

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