Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Biotechnol Lett

Search In Journal Title:

Abbravation: Biotechnology Letters

Search In Journal Abbravation:

Publisher

Springer Netherlands

Search In Publisher:

DOI

10.1007/bf02237059

Search In DOI:

ISSN

1573-6776

Search In ISSN:
Search In Title Of Papers:

Isolation and functional characterization of polyu

Authors: Sun Hee Kim Jong Bum Kim Yo Soon Jang So Yun Kim Kyung Hee Roh Hyun Uk Kim KyeongRyeol Lee JongSug Park
Publish Date: 2011/12/24
Volume: 34, Issue: 2, Pages: 261-268
PDF Link

Abstract

To identify the genes encoding fatty acid elongases for the biosynthesis of polyunsaturated fatty acids PUFAs we isolated a cDNA via degenerate PCR and RACEPCR from Acanthopagrus schlegelii with a high similarity to the ELOVL5like elongases of mammals and fishes This gene is termed AsELOVL5 and encodes a 294 amino acid protein When AsELOVL5 was expressed in Saccharomyces cerevisiae it conferred an ability to elongate γlinolenic acid 183 n−6 to dihomoγlinolenic acid 203 n−6 In addition the transformed cells converted arachidonic acid 204 n−6 and eicosapentaenpic acid 205 n−3 to docosatetraenoic acid 224 n−6 and docosapentaenoic acid 225 n−3 respectively These results indicate that the AsELOVL5 gene encodes a longchain fatty acid elongase capable of elongating C18Δ6/C20Δ5 but not C22 PUFA substratesThis work was supported by grants from the National Academy of Agricultural Science PJ006745 the Rural Development Administration Republic of Korea SH Kim was supported by a 2011 Postdoctoral Fellowship Program of National Academy of Agricultural Science the Rural Development Administration Republic of Korea

Keywords:

References

.
Search In Abstract Of Papers:
Other Papers In This Journal:
  1. Direct detection, cloning and characterization of a glucoside hydrolase from forest soil
  2. Isolation of sensitive nisin-sensing GFP uv bioassay Lactococcus lactis strains using FACS
  3. Silk screen based dual spin-filter module for perfusion culture of adherent and non-adherent mammalian cells
  4. Production of β-carotene by expressing a heterologous multifunctional carotene synthase in Yarrowia lipolytica
  5. Biohydrogen production by Enterobacter cloacae and Citrobacter freundii in carrier induced granules
  6. High-yield production of short chain length poly(ε- l -lysine) consisting of 5–20 residues by Streptomyces aureofaciens , and its antimicrobial activity
  7. Isolation and characterization of a novel ε-caprolactam-degrading microbe, Acinetobacter calcoaceticus , from industrial wastewater by chemostat-enrichment
  8. Specificity of a DNA-based (DNAzyme) peroxidative biocatalyst
  9. Enzymatic transformation of nitro-aromatic compounds by a flavin-free NADH azoreductase from Lysinibacillus sphaericus
  10. Expression of truncated tobacco osmotin in Escherichia coli : purification and antifungal activity
  11. In vitro generation of type-II pneumocytes can be initiated in human CD34 + stem cells
  12. Electrospun silk fibroin mat enhances tendon-bone healing in a rabbit extra-articular model
  13. Remediation of the effect of adding cyanides on an algal/bacterial treatment of a mixture of organic pollutants in a continuous photobioreactor
  14. Concave microwell plate facilitates chondrogenesis from mesenchymal stem cells
  15. Expression, characterization of a novel nitrilase PpL19 from Pseudomonas psychrotolerans with S -selectivity toward mandelonitrile present in active inclusion bodies
  16. Improved serum stability and biophysical properties of siRNAs following chemical modifications
  17. Lentiviral vector-derived shRNAs confer enhanced suppression of Semliki forest virus replication in BHK-21 cells compared to shRNAs expressed from plasmids
  18. Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice
  19. Aerobic microbial manufacture of nanoscale selenium: exploiting nature’s bio-nanomineralization potential
  20. From bio-mineralisation to fuel cells: biomanufacture of Pt and Pd nanocrystals for fuel cell electrode catalyst
  21. Over-production of a glycoside hydrolase family 50 β-agarase from Agarivorans sp. JA-1 in Bacillus subtilis and the whitening effect of its product
  22. Isotopic labeling of the heme cofactor in cytochrome p450 and other heme proteins
  23. pEGFP-T, a novel T-vector for the direct, unidirectional cloning and analysis of PCR-amplified promoters
  24. Specific monitoring of cardiomyogenic and endothelial differentiation by dual promoter-driven reporter systems in bone marrow mesenchymal stem cells
  25. Lipase-catalyzed simultaneous biosynthesis of biodiesel and glycerol carbonate from corn oil in dimethyl carbonate
  26. Towards β-globin gene-targeting with integrase-defective lentiviral vectors
  27. Enhanced bacterial expression of several mammalian cytochrome P450s by codon optimization and chaperone coexpression
  28. Ultraviolet-B radiation improves astaxanthin accumulation in green microalga Haematococcus pluvialis
  29. NahK/GlmU fusion enzyme: characterization and one-step enzymatic synthesis of UDP- N -acetylglucosamine
  30. A one-pot system for production of l -2-aminobutyric acid from l -threonine by l -threonine deaminase and a NADH-regeneration system based on l -leucine dehydrogenase and formate dehydrogenase
  31. Relationship between tissue plasminogen activator production and specific growth rate in Chinese Hamster Ovary cells cultured in mannose at low temperature
  32. Generation of Rm21LG transgenic mice: a powerful tool to generate conditional overexpression of miR-21 that is involved in oncogenesis
  33. Production of biomass and recombinant human-like collagen in Escherichia coli processes with different CO 2 pulses
  34. Screening of Aspergillus -derived FA D -glucose dehydrogenases from fungal genome database
  35. Well-defined differentiation of hesc-derived hemangioblasts by embryoid body formation without enzymatic treatment
  36. Single-colour flow cytometric assay to determine NK cell-mediated cytotoxicity and viability against non-adherent human tumor cells
  37. cry1 genes from Bacillus thuringiensis : specificity determination and implications for primer design
  38. Taxol-induced alteration of intracellular amino-acid profile related to human cervical carcinoma HeLa cell death
  39. Enhanced thermotolerance for ethanol fermentation of Saccharomyces cerevisiae strain by overexpression of the gene coding for trehalose-6-phosphate synthase
  40. High pressure treatment under subfreezing temperature results in drastic inactivation of enveloped and non-enveloped viruses
  41. Comparative analysis of young panicle proteome in thermo-sensitive genic male-sterile rice Zhu-1S under sterile and fertile conditions
  42. Multiparameter flow cytometry for the characterisation of extracellular markers on human mesenchymal stem cells
  43. Mycoparasitism studies of Trichoderma harzianum against Sclerotinia sclerotiorum : evaluation of antagonism and expression of cell wall-degrading enzymes genes
  44. Surfactin C inhibits Mycoplasma hyopneumoniae -induced transcription of proinflammatory cytokines and nitric oxide production in murine RAW 264.7 cells
  45. Refolding of denatured lysozyme by water-in-oil microemulsions of sucrose fatty acid esters
  46. Engineering the thermostability of a xylanase from Aspergillus oryzae by an enhancement of the interactions between the N -terminus extension and the β-sheet A2 of the enzyme
  47. Porcine testicular extract inhibits T cell proliferation by blocking cell cycle transition from G 1 phase to S phase
  48. Helicase translocation assay method using avidin and biotinylated nucleotides
  49. Site-directed modification of the adenylation domain of the fusaricidin nonribosomal peptide synthetase for enhanced production of fusaricidin analogs
  50. Biotransformation of artemisinin using cell suspension cultures of Catharanthus roseus (L.) G.Don and Lavandula officinalis L.
  51. Online monitoring of oxygen in spinner flasks
  52. Attachment of Acidithiobacillus ferrooxidans onto different solid substrates and fitting through Langmuir and Freundlich equations
  53. Endoglucanase enzymatic modification of kraft pulp during recycling
  54. Conjugation of DNA with protein using His-tag chemistry and its application to the aptamer-based detection system
  55. Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion: case of a nonglycosylated mutant of miraculin, a taste-modifying protein
  56. Colonization of sorghum and wheat by seed inoculation with Gluconacetobacter diazotrophicus
  57. Overexpression of NgAUREO1, the gene coding for aurechrome 1 from Nannochloropsis gaditana , into Saccharomyces cerevisiae leads to a 1.6-fold increase in lipid accumulation
  58. Plate in situ hybridization (PISH) as a time and cost effective RNA expression assay to study phenotypic heterogeneity in a population of cultured murine cells at single cell resolution
  59. A simple and rapid approach to manipulate pseudorabies virus genome by CRISPR/Cas9 system
  60. Purification of a recombinant histidine-tagged lactate dehydrogenase from the malaria parasite, Plasmodium vivax, and characterization of its properties
  61. Anti-Prelog Reduction of Prochiral Carbonyl Compounds by Oenococcus oeni in a Biphasic System
  62. Production of 1,3-propanediol by Klebsiella pneumoniae from glycerol broth
  63. Changing the specificity of α-amino acid ester hydrolase toward para -hydroxyl cephalosporins synthesis by site-directed saturation mutagenesis
  64. Constructing Bacillus thuringiensis strain that co-expresses Cry2Aa and chitinase
  65. RETRACTED ARTICLE: Discovery of a novel inhibitory mechanism of kaempferol on TGF-β ligand-receptor interaction by single-molecule techniques
Search Result: