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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1002/oms.1210020706

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1573-6776

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Production of βcarotene by expressing a heterolog

Authors: Shuliang Gao Yangyang Tong Li Zhu Mei Ge Yu Jiang Daijie Chen Sheng Yang
Publish Date: 2017/03/13
Volume: 39, Issue: 6, Pages: 921-927
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Abstract

To obtain functional expression of a heterologous multifunctional carotene synthase containing phytoene synthase phytoene dehydrogenase and lycopene βcyclase activities encoded by carS from Schizochytrium sp in order to allow Yarrowia lipolytica to produce βcaroteneTo increase the integration efficiency of a 38 kb carS under the control of P GPD promoter with a 2 kb selection marker ura3 along with a geranylgeranyl diphosphate synthase GGS1 expression cassette ~10 kb in total was inserted into the Y lipolytica chromosome and the DNA assembler method was combined with double chromosomal deletions of ku70 and ku80 This method resulted in a 134fold increase in integration efficiency compared with the original method reaching 63 10/16 The resulting recombinant Y lipolytica produced 041 mg βcarotene per g dry cell weight while the wild type did not produce any indicating the functionality of the multifunctional carotene synthase in Y lipolyticaExpression of GGS1 and a multifunctional carotene synthase from Schizochytrium sp in Y lipolytica led to βcarotene production DNA assembler efficiency was greatly increased by the deletion of ku70 and ku80 which resulted in decreased in vivo nonhomologous endjoining NHEJ in Y lipolytica

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