Paper Search Console

Home Search Page About Contact

Journal Title

Title of Journal: Biotechnol Lett

Search In Journal Title:

Abbravation: Biotechnology Letters

Search In Journal Abbravation:

Publisher

Springer Netherlands

Search In Publisher:

DOI

10.1002/chin.198746053

Search In DOI:

ISSN

1573-6776

Search In ISSN:
Search In Title Of Papers:

Refolding of denatured lysozyme by waterinoil mi

Authors: Hidetaka Noritomi Tsubasa Takasugi Satoru Kato
Publish Date: 2007/12/08
Volume: 30, Issue: 4, Pages: 689-693
PDF Link

Abstract

Waterinoil w/o microemulsion of sucrose fatty acid ester was used to renature denatured hen egg white lysozyme without aggregation After lysozyme was denatured in 5 M guanidine hydrochloride for 24 h the resultant denatured lysozyme was held in the microemulsion overnight at 25°C Renatured lysozyme was transferred from the microemulsion phase to the recovery aqueous phase by conventional liquidliquid extraction The enzymatic activity of the recovered lysozyme was 93

Keywords:

References

.
Search In Abstract Of Papers:
Other Papers In This Journal:
  1. Direct detection, cloning and characterization of a glucoside hydrolase from forest soil
  2. Isolation of sensitive nisin-sensing GFP uv bioassay Lactococcus lactis strains using FACS
  3. Silk screen based dual spin-filter module for perfusion culture of adherent and non-adherent mammalian cells
  4. Production of β-carotene by expressing a heterologous multifunctional carotene synthase in Yarrowia lipolytica
  5. Biohydrogen production by Enterobacter cloacae and Citrobacter freundii in carrier induced granules
  6. High-yield production of short chain length poly(ε- l -lysine) consisting of 5–20 residues by Streptomyces aureofaciens , and its antimicrobial activity
  7. Isolation and characterization of a novel ε-caprolactam-degrading microbe, Acinetobacter calcoaceticus , from industrial wastewater by chemostat-enrichment
  8. Specificity of a DNA-based (DNAzyme) peroxidative biocatalyst
  9. Enzymatic transformation of nitro-aromatic compounds by a flavin-free NADH azoreductase from Lysinibacillus sphaericus
  10. Expression of truncated tobacco osmotin in Escherichia coli : purification and antifungal activity
  11. Isolation and functional characterization of polyunsaturated fatty acid elongase (AsELOVL5) gene from black seabream ( Acanthopagrus schlegelii )
  12. In vitro generation of type-II pneumocytes can be initiated in human CD34 + stem cells
  13. Electrospun silk fibroin mat enhances tendon-bone healing in a rabbit extra-articular model
  14. Remediation of the effect of adding cyanides on an algal/bacterial treatment of a mixture of organic pollutants in a continuous photobioreactor
  15. Concave microwell plate facilitates chondrogenesis from mesenchymal stem cells
  16. Expression, characterization of a novel nitrilase PpL19 from Pseudomonas psychrotolerans with S -selectivity toward mandelonitrile present in active inclusion bodies
  17. Improved serum stability and biophysical properties of siRNAs following chemical modifications
  18. Lentiviral vector-derived shRNAs confer enhanced suppression of Semliki forest virus replication in BHK-21 cells compared to shRNAs expressed from plasmids
  19. Oral vaccine of Lactococcus lactis harbouring pandemic H1N1 2009 haemagglutinin1 and nisP anchor fusion protein elevates anti-HA1 sIgA levels in mice
  20. Aerobic microbial manufacture of nanoscale selenium: exploiting nature’s bio-nanomineralization potential
  21. From bio-mineralisation to fuel cells: biomanufacture of Pt and Pd nanocrystals for fuel cell electrode catalyst
  22. Over-production of a glycoside hydrolase family 50 β-agarase from Agarivorans sp. JA-1 in Bacillus subtilis and the whitening effect of its product
  23. Isotopic labeling of the heme cofactor in cytochrome p450 and other heme proteins
  24. pEGFP-T, a novel T-vector for the direct, unidirectional cloning and analysis of PCR-amplified promoters
  25. Specific monitoring of cardiomyogenic and endothelial differentiation by dual promoter-driven reporter systems in bone marrow mesenchymal stem cells
  26. Lipase-catalyzed simultaneous biosynthesis of biodiesel and glycerol carbonate from corn oil in dimethyl carbonate
  27. Towards β-globin gene-targeting with integrase-defective lentiviral vectors
  28. Enhanced bacterial expression of several mammalian cytochrome P450s by codon optimization and chaperone coexpression
  29. Ultraviolet-B radiation improves astaxanthin accumulation in green microalga Haematococcus pluvialis
  30. NahK/GlmU fusion enzyme: characterization and one-step enzymatic synthesis of UDP- N -acetylglucosamine
  31. A one-pot system for production of l -2-aminobutyric acid from l -threonine by l -threonine deaminase and a NADH-regeneration system based on l -leucine dehydrogenase and formate dehydrogenase
  32. Relationship between tissue plasminogen activator production and specific growth rate in Chinese Hamster Ovary cells cultured in mannose at low temperature
  33. Generation of Rm21LG transgenic mice: a powerful tool to generate conditional overexpression of miR-21 that is involved in oncogenesis
  34. Production of biomass and recombinant human-like collagen in Escherichia coli processes with different CO 2 pulses
  35. Screening of Aspergillus -derived FA D -glucose dehydrogenases from fungal genome database
  36. Well-defined differentiation of hesc-derived hemangioblasts by embryoid body formation without enzymatic treatment
  37. Single-colour flow cytometric assay to determine NK cell-mediated cytotoxicity and viability against non-adherent human tumor cells
  38. cry1 genes from Bacillus thuringiensis : specificity determination and implications for primer design
  39. Taxol-induced alteration of intracellular amino-acid profile related to human cervical carcinoma HeLa cell death
  40. Enhanced thermotolerance for ethanol fermentation of Saccharomyces cerevisiae strain by overexpression of the gene coding for trehalose-6-phosphate synthase
  41. High pressure treatment under subfreezing temperature results in drastic inactivation of enveloped and non-enveloped viruses
  42. Comparative analysis of young panicle proteome in thermo-sensitive genic male-sterile rice Zhu-1S under sterile and fertile conditions
  43. Multiparameter flow cytometry for the characterisation of extracellular markers on human mesenchymal stem cells
  44. Mycoparasitism studies of Trichoderma harzianum against Sclerotinia sclerotiorum : evaluation of antagonism and expression of cell wall-degrading enzymes genes
  45. Surfactin C inhibits Mycoplasma hyopneumoniae -induced transcription of proinflammatory cytokines and nitric oxide production in murine RAW 264.7 cells
  46. Engineering the thermostability of a xylanase from Aspergillus oryzae by an enhancement of the interactions between the N -terminus extension and the β-sheet A2 of the enzyme
  47. Porcine testicular extract inhibits T cell proliferation by blocking cell cycle transition from G 1 phase to S phase
  48. Helicase translocation assay method using avidin and biotinylated nucleotides
  49. Site-directed modification of the adenylation domain of the fusaricidin nonribosomal peptide synthetase for enhanced production of fusaricidin analogs
  50. Biotransformation of artemisinin using cell suspension cultures of Catharanthus roseus (L.) G.Don and Lavandula officinalis L.
  51. Online monitoring of oxygen in spinner flasks
  52. Attachment of Acidithiobacillus ferrooxidans onto different solid substrates and fitting through Langmuir and Freundlich equations
  53. Endoglucanase enzymatic modification of kraft pulp during recycling
  54. Conjugation of DNA with protein using His-tag chemistry and its application to the aptamer-based detection system
  55. Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion: case of a nonglycosylated mutant of miraculin, a taste-modifying protein
  56. Colonization of sorghum and wheat by seed inoculation with Gluconacetobacter diazotrophicus
  57. Overexpression of NgAUREO1, the gene coding for aurechrome 1 from Nannochloropsis gaditana , into Saccharomyces cerevisiae leads to a 1.6-fold increase in lipid accumulation
  58. Plate in situ hybridization (PISH) as a time and cost effective RNA expression assay to study phenotypic heterogeneity in a population of cultured murine cells at single cell resolution
  59. A simple and rapid approach to manipulate pseudorabies virus genome by CRISPR/Cas9 system
  60. Purification of a recombinant histidine-tagged lactate dehydrogenase from the malaria parasite, Plasmodium vivax, and characterization of its properties
  61. Anti-Prelog Reduction of Prochiral Carbonyl Compounds by Oenococcus oeni in a Biphasic System
  62. Production of 1,3-propanediol by Klebsiella pneumoniae from glycerol broth
  63. Changing the specificity of α-amino acid ester hydrolase toward para -hydroxyl cephalosporins synthesis by site-directed saturation mutagenesis
  64. Constructing Bacillus thuringiensis strain that co-expresses Cry2Aa and chitinase
  65. RETRACTED ARTICLE: Discovery of a novel inhibitory mechanism of kaempferol on TGF-β ligand-receptor interaction by single-molecule techniques
Search Result: