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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1016/0022-5088(64)90085-2

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1573-6776

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Relationship between tissue plasminogen activator

Authors: Julio Berrios Alvaro DíazBarrera Consuelo Bazán Claudia Altamirano
Publish Date: 2009/06/23
Volume: 31, Issue: 10, Pages: 1493-1497
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Abstract

Chinese hamster ovary CHO cells producing human recombinant tissue plasminogen activator tPA were grown with mannose 5 20 and 40 mM instead of glucose at 31 33 and 37°C The highest tPA concentration 15 mg l−1 at 144 h of cultivation and tPA specific production rate 47 ng 10−6 cell h−1 were obtained at 31°C and 40 mM mannose Regardless of the temperature or mannose concentration used an inverse relationship between the specific growth rate and tPA specific production rate was observed suggesting that tPA production rate would be directly controlled by the growth rate

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