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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1007/bf02244747

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1573-6776

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Constructing Emphasis Type="Italic"Bacillus thur

Authors: Shengbiao Hu Xu Zhang Yusheng Li Xuezhi Ding Xiaofeng Hu Qi Yang Liqiu Xia
Publish Date: 2013/03/21
Volume: 35, Issue: 7, Pages: 1045-1051
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Abstract

A triple recombineering technique was used with plasmid pHT315 to produce pHTEC a construct carrying chitinase and cry2Aa genes from Bacillus thuringiensis subsp kurstaki 40718 Transformation of wildtype B thuringiensis strain HD73 and the acrystalliferous strain CryB with pHTEC resulted in the recovery of recombinant strains that expressed Cry2Aa as cubic crystals in the cell pellet and soluble chitinase protein The toxicity of HD73 pHTEC against Helicoverpa armigera larvae increased sevenfold when compared with HD73 pHT315 harboring pHT315 vector The triple recombineering protocol was optimized by comparing recombination efficacy mediated by RecE/RecT and Redα/Redβ and by using singlestrand DNA as substrateThis investigation was supported by National Natural Science Foundation of China 31200004 Specialized Research Fund for the Doctoral Program of Higher Education from the Ministry of Education of China 2011430620005 Hunan Provincial Natural Science Foundation of China 10JJ6042 Scientific Research Fund of Hunan Provincial Education Department 10K041

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