Authors: Kazushige Mori Mitsuharu Nakajima Katsuhiro Kojima Koudai Murakami Stefano Ferri Koji Sode
Publish Date: 2011/07/12
Volume: 33, Issue: 11, Pages: 2255-
Abstract
Aspergillusderived FADdependent glucose dehydrogenases FADGDHs were screened from fungal genomic databases primarily by searching for putative homologues of the Aspergillus nigerderived glucose oxidase GOD Focusing on a GOD activesite motif putative proteins annotated as belonging to the glucose methanol choline GMC oxidoreductase family were selected Phylogenetic analysis of these putative proteins produced a GOD clade which includes the A niger and Penicillium amagasakiens GODs and a second clade made up of putative proteins showing 30–40 homology with GOD The genes encoding the proteins from the second clade were functionally expressed in Escherichia coli resulting in dyemediated glucose dehydrogenase GDH activity but not GOD activity These results suggest that the putative proteins belonging to the second clade are FADGDHs The 3D structure models of these FADGDHs were compared with the 3D structure of GOD
Keywords: