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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1016/0008-8749(85)90010-3

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1573-6776

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Overexpression of NgAUREO1 the gene coding for au

Authors: Yijiang Huang Ling Wang Minggang Zheng Li Zheng Yanli Tong Yan Li
Publish Date: 2013/10/27
Volume: 36, Issue: 3, Pages: 575-579
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Abstract

Aureochrome1 AUREO1 is a transcription factor that is induced by blue light and controls branching of Vaucheria frigida We have cloned the gene NgAUREO1 coding for AUREO1 from Nannochloropsis gaditana and report that the lipid content in recombinant Saccharomyces cerevisiae was 16fold more than in wildtype S cerevisiae 63  lipid increased to 10  Overexpression of AUREO1 in S cerevisiae upregulated the expression of acetylCoA carboxylase and acylCoAdiacylglycerol acyltransferase but downregulated the expression of longchainacyl CoA synthetase This enhanced the accumulation of lipid This study highlights a novel function of AUREO1 and allows a better understanding of the regulation mechanism of fatty acid metabolismThis work was supported by the National Natural Science Foundation of China Grant No 41106148 31170109 31270408 Provincial Natural Science Foundation of Shandong Grant No ZR2010DQ013 2011GHY11533 and Marine Renewable Energy Funds Project Grant No 201305022 GHME2001SW02

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