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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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1573-6776

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Generation of Rm21LG transgenic mice a powerful t

Authors: Tao Du Junshuang Jia Xiaolin Lin Raoying Xie Jing Li Dong Xiao Kang Xu
Publish Date: 2013/09/26
Volume: 36, Issue: 1, Pages: 9-20
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Abstract

miR21 is highly expressed in a variety of cancers suggesting that it might play a role in the process of oncogenesis as supported by it directly causing preB cell lymphomas in transgenic mice Rm21LG transgenic mice for the conditional coexpression of miR21 and luciferase Luc mediated by Cre/lox P system were generated The homozygous Rm21LG transgenic mice were visually and readily characterized immediately after birth by wholebody fluorescence imaging More importantly miR21 and Luc were successfully activated in the liver of Rm21LG/AlbCre doubletransgenic mice demonstrating that Rm21LG conditional transgenic system could work in a Credependent manner The combined use of this conditional miR21 transgenic mouse line various cell/tissuespecific Cre mouse lines and bioluminescence imaging will be a valuable tool in vivo to uncover the functions of miR21 as oncomiR in initiating tumorsWe thank Prof Manuela MartinsGreen University of California for generously providing pRLG plasmid This work was supported by the National Natural Science Foundation of China Grant Nos 81172587 81100573 the Natural Science Foundation of Guangdong Province of China Grant No S2012010009212 and the Fundamental Research Funds for the Central Universities of China Grant No 10ykpy21

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