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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1002/jps.2600631208

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1573-6776

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Overproduction of a glycoside hydrolase family 50

Authors: DongGeun Lee Min Kyung Jang OkHee Lee Nam Young Kim SeongA Ju SangHyeon Lee
Publish Date: 2008/01/10
Volume: 30, Issue: 5, Pages: 911-918
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Abstract

The gene for βagarase of an Agarivorans sp JA1 was expressed in Bacillus subtilis strain DB104 for efficient and economical massproduction of the enzyme We isolated 360 mg protein with a specific activity of 201 U/mg from the culture broth The efficiency of production was approximately 130fold higher than that in E coli The enzyme produced neoagarohexaose neoagarotetraose and neoagarobiose from agar Neoagarooligosaccharides produced by the enzyme had a whitening effect and inhibited tyrosinase activity in the murine melanoma cell line B16F10 Neoagarooligosaccharides were not cytotoxic to B16F10 or normal cells βAgarase could therefore be a good whitening cosmetic additive

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