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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1007/bf02406736

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ISSN

1573-6776

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Concave microwell plate facilitates chondrogenesis

Authors: JiYun Ko GunIl Im
Publish Date: 2016/08/17
Volume: 38, Issue: 11, Pages: 1967-1974
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Abstract

Pellets cultured in concave microwell plates had a significantly higher level of GAG per DNA content and greater proteoglycan content than those cultured in tubes at day 7 and 14 Three chondrogenic markers SOX9 COL2A1 and aggrecan showed significantly higher expression in pellets cultured in concave microwell plates than those cultured in tubes at day 7 and 14 At day 21 there was not a significant difference in the expression of these markers COL10A1 the typical hypertrophy marker was significantly lower in concave microwell plates during the whole culture period Runx2 a marker of hypertrophy and osteogenesis was significantly lower at day 7 in pellets cultured in concave microwell plates than those cultured in tubes

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