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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1016/0741-5214(91)90319-P

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1573-6776

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High pressure treatment under subfreezing temperat

Authors: T Kishida FD Cui E Ohgitani F Gao K Hayakawa O Mazda
Publish Date: 2013/04/23
Volume: 35, Issue: 8, Pages: 1297-1301
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Abstract

Some viruses are sensitive to high pressure The freezepressure generation method FPGM applies pressure as high as 250 MPa on a substance simply by freezing a pressureresistant reservoir in which the substance is immersed in water Here we examined whether the FPGM successfully inactivates herpes simplex virus type 1 HSV1 an enveloped DNA virus belonging to the human Herpesviridae and encephalomyocarditis virus EMCV an envelopefree RNA virus belonging to the Picornaviridae After the treatment HSV1 drastically reduced the ability to form plaque in Vero cells in vitro as well as to kill mice in vivo EMCV that had been pressurized failed to proliferate in HeLa cells and induce interferon response The results suggest that the FPGM provides a feasible procedure to inactivate a broad spectrum of viruses

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