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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1007/s00406-013-0437-9

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1573-6776

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Production of biomass and recombinant humanlike c

Authors: Wen J Xue Dai D Fan Longan Shang Chen H Zhu Xiao X Ma Yuan Y Yu
Publish Date: 2008/10/16
Volume: 31, Issue: 2, Pages: 221-226
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Abstract

The evolution of CO2 in a fedbatch culture of recombinant Escherichia coli containing humanlike collagen HLC cDNA was determined with an O2enriched air supply 40 v/v in a 128 l fermentor a maximum CO2 concentration of 127 in the effluent gas was detected The CO2 pulse injection experiments showed that 1 a 20 CO2 pulse introduced in the batch cultivation phases inhibited cell growth but if introduced in the fedbatch cultivation phases slightly stimulated growth and 2 CO2 inhibited HLC expression only in the expression phase where the final HLC concentration decreased by 34 under a 3 h 20 CO2 pulse The higher the CO2 concentration and/or the longer the duration of the CO2 pulse the stronger the stimulatory or inhibitory effectsThis study was financially supported by the National High Technology Research and Development Program of China 863 Program 2006AA02Z246 and the National Natural Science Foundation of China 20776119 Especially we are grateful to Northwest University for granting the Graduate Crossdiscipline Funds 07YJC12 to support this study

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