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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1007/bf00636528

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1573-6776

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Enhanced thermotolerance for ethanol fermentation

Authors: MingZhe An YueQin Tang Kanako Mitsumasu ZeShen Liu Morimura Shigeru Kida Kenji
Publish Date: 2011/03/06
Volume: 33, Issue: 7, Pages: 1367-1374
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Abstract

The effect of overexpression of the trehalose6phosphate T6P synthase gene TPS1 on ethanol fermentation of Saccharomyces cerevisiae has been studied at 30 and 38°C The activity of T6P synthase and the accumulation of trehalose during ethanol fermentation were significantly improved by overexpression of TPS1 and especially at 38°C Ethanol produced by transformants with and without TPS1 gene overexpression at 38°C was approx 60 and 37 g/l respectively The fermentation efficiency of transformants with TPS1 gene overexpression at 38°C was similar to that at 30°C The critical growth temperature was increased from 36 to 42°C by TPS1 gene overexpression These results indicated that overexpression of the TPS1 gene had a beneficial effect on the fermentation capacity of the title yeast strain at high temperatures

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