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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1007/bf03168588

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ISSN

1573-6776

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Comparative analysis of young panicle proteome in

Authors: Xiaojuan Xiao Yuanzhu Yang Yuejun Yang Jianzhong Lin Dongying Tang Xuanming Liu
Publish Date: 2008/10/16
Volume: 31, Issue: 1, Pages: 157-161
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Abstract

Proteome analysis was carried out to identify the young panicle proteins during different developmental stages under sterile and fertile conditions Based on spot quantity and quality 50 protein spots were analyzed by matrix associated laser desorption/ionization time of flight mass spectrometry MALDITOFMS and 20 spots were identified Most of these proteins are closely associated with energy metabolism protein biosynthesis cell wall formation and stress responses which are essential cell activities to the pollen development Gene expression analysis of three different proteins by semiquantitative RTPCR showed that the mRNA level was not correlated exactly with the protein level

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