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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1016/j.bbmt.2006.12.115

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1573-6776

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Welldefined differentiation of hescderived heman

Authors: ChangHyun Gil Jiheon Lee Joseph Seo SoonJung Park Zewon Park Jumi Kim ARa Jung WonYoung Lee JongSoo Kim SungHwan Moon HoonTaek Lee HyungMin Chung
Publish Date: 2015/02/21
Volume: 37, Issue: 6, Pages: 1315-1322
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Abstract

Human hemangioblasts exist only during the early embryonic developmental stage thereby limiting the adult cellular source from which to obtain such cells for study To overcome this hemangioblast studies have focused on utilizing human embryonic stem cell hESC derivatives but current methods are cellline dependent Single cell dissociation of a hESC colony quickly led to cell death in most hESC lines due to enzyme treatment which in turn reduced induction potential and hemangioblast differentiation efficiency Therefore we sought to effectively improve the process of cell dissociation that is adaptable to various hESC lines and increase the initial induction potential of embryoid body hEB As a result we determined an effective cell dissociation method through a comparison study involving various reagents which demonstrated successful dissociation regardless of cell line and enhanced hemangioblast differentiation efficiencyThis study was supported by grant 2011–0019487 from the Bio Medical Technology Development Program of the National Research Foundation NRF and supported by a grant PJ00995602 PJ00933303 from the NextGeneration BioGreen 21 Program of Rural Development Administration funded by the Korean government

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