Authors: Suman Patel Rashmi Gaur Priyanka Verma Rajendra S Bhakuni Archana Mathur
Publish Date: 2010/04/06
Volume: 32, Issue: 8, Pages: 1167-1171
Abstract
Artemisinin an antimalarial compound at 5 mg/40 ml was transformed by cell suspension cultures of Catharanthus roseus L GDon and Lavandula officinalis L into deoxyartemisinin with yields 78 393 mg deoxyartemisinin from 5 mg artemisinin Maximum conversion 786 and 78 occurred after 6 and 7 days of adding artemisinin to 20 and 9 days old cultures of C roseus and L officinalis respectively The procedure was scaled up by and 500 mg artemisinin was transformed into 390 mg deoxyartemisinin Addition of artemisinin at the beginning of the culture cycle resulted in 50 reduction in dry biomass production with no bioconversion Conversion of artemisinin occurred intracellularly followed by leaching of the product into the mediumWe are thankful to Director CIMAP Lucknow for providing the necessary facilities and constant encouragement to carry out this work The work is carried out under a CSIR network programme NWP09 on “Chemical and Biological Transformations of Plant Compounds for Production of Valueadded Products of Therapeutic/Aroma Value”
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