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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1007/978-0-387-49493-7_11

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1573-6776

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Expression of truncated tobacco osmotin in Emphas

Authors: YwhMin Tzou TungShi Huang Kevin W Huggins Bryan A Chin Amarat H Simonne Narendra K Singh
Publish Date: 2010/11/03
Volume: 33, Issue: 3, Pages: 539-543
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Abstract

Expression of recombinant tobacco osmotin rOSM in Escherichia coli inclusion bodies has been achieved The optimal pH for rOSM expression in ZYM 505 medium is 70 at OD650 of 15 of culture growth The rOSM from the inclusion body was extracted with 8 M urea and purified using CMcellulose and cobaltagarose bead affinity chromatography to a high purity Approximately 80 of the rOSM remained bound to CMcellulose and Cobaltagarose beads after initial elution The yield of purified rOSM was between 40 and 50 mg from 2 l of culture Repeated elution of protein from CMcellulose and Coagarose increased the yield of rOSM to 200 mg from 2 l culture The purified rOSM showed variable antifungal activities against two pathogenic yeast strains Cryptococcus neoformans Candida albicans and nonpathogenic strains Saccharomyces cerevisiae and Pichia methanolica

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