Authors: Rogério Fraga Troian Andrei Stecca Steindorff Marcelo Henrique Soller Ramada Walquiria Arruda Cirano José Ulhoa
Publish Date: 2014/06/26
Volume: 36, Issue: 10, Pages: 2095-2101
Abstract
Trichoderma spp are known for their biocontrol activity against several plant pathogens A specific isolate of Trichoderma harzianum 303/02 has the potential to inhibit the growth of Sclerotinia sclerotiorum an important agent involved in several crop diseases In this study the interaction between T harzianum 303/02 and mycelia sclerotia and apothecia of S sclerotiorum was studied by scanning electron microscopy RTqPCR was used to examine the expression of 11 genes potentially involved in biocontrol T harzianum 303/02 parasitizes S sclerotiorum by forming branches that coil around the hyphae The fungus multiplied abundantly at the sclerotia and apothecia surface forming a dense mycelium that penetrated the inner surface of these structures The levels of gene expression varied according to the type of structure with which T harzianum was interacting The data also showed the presence of synergistic action between the cellwall degrading enzymesThis work was funded by FINEP Research and Projects Financing the National Council for Scientific and Technological Development CNPq and the State of Goiás Research Foundation FAPEGO TFQ was supported by a scholarship from CNPq We kindly thank Dr Robert Poque for reading the manuscript and providing suggestions
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