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Title of Journal: Biotechnol Lett

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Abbravation: Biotechnology Letters

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Springer Netherlands

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DOI

10.1007/3-540-27218-6_11

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1573-6776

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Conjugation of DNA with protein using Histag chem

Authors: Josui Shimada Tatsuo Maruyama Takuya Hosogi Jo Tominaga Noriho Kamiya Masahiro Goto
Publish Date: 2008/07/05
Volume: 30, Issue: 11, Pages: 2001-2006
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Abstract

We propose a novel method to prepare a DNA–protein conjugate using histidinetag Histag chemistry OligoDNA was modified with nitrilotriacetate NTA which has high affinity to a Histag on recombinant protein via the complexation of Ni2+ Investigations using a microplate which displayed a complementary DNAstrand revealed that a NTAmodified DNA–protein conjugate was formed and immobilized in the presence of Ni2+ on the microplate We then adopted alkaline phosphatase AP as a model protein and application of the DNA–AP conjugate was demonstrated in a thrombin aptamerbased detection system with a detection limit of approximately 10 nM

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