Authors: Munish Kumar Sudhir K Agarwal Sudhir K Goel
Publish Date: 2008/11/14
Volume: 322, Issue: 1-2, Pages: 73-79
Abstract
Lung cancer LC is the leading cause of cancerrelated mortality in developing as well as developed countries Life style choices particularly tobacco smoking have been implicated as the main cause in the development of the LC Despite the fact that majority cases of the LC occur among smokers only 1–15 of smokers develop LC In the present study we have explored the role of genetic polymorphism smoking habit and their association to LC in a cohort of north Indian population The polymorphic genes explored were CYP1A1 GSTM1 GSTP1 and GSTT1 using techniques of Polymerase chain reaction PCR Restriction Fragment Length Polymorphism RFLP Real Time PCR RT PCR and gene sequencing Genetic polymorphism was analysed in 253 normal participants control and 93 LC patients originating from Lucknow India Data were compared using odds ratio and Fisher Exact Test We found that smoking increases the susceptibility to LC threefold OR = 29 95 CI 09–28 The most significant risk for LC OR = 32 95 CI 07–38 was found in the association of the homozygous variant of CYP1A1 gene at A2455G base change at Exon 7 Val/Val genotype There was a marginally significant association between LC and GSTT1 null genotype OR = 13 95 CI 10–17 while no significant risk association was found between GSTP1 polymorphism and LC The present study demonstrates that the presence of null genotype of GSTM1/GSTT1 taken together with CYP1A1 Val/Val genotype increases the susceptibility to LC eightfold in comparison to CYP1A1 Ile/Ile and GSTM1/ GSTT1 genotype
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