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Title of Journal: Mol Cell Biochem

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Abbravation: Molecular and Cellular Biochemistry

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Springer US

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DOI

10.1016/1359-6462(95)00681-8

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1573-4919

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Inhibition of p90 ribosomal S6 kinase attenuates c

Authors: Nabeel Abdulrahman Maiy Jaballah Noufira Poomakkoth Sadaf Riaz Somaia Abdelaziz Aya Issa Fatima Mraiche
Publish Date: 2016/05/28
Volume: 418, Issue: 1-2, Pages: 21-29
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Abstract

p90 ribosomal S6 kinase p90RSK constitutes a family of serine/threonine kinases that have been shown to be involved in cell proliferation of various malignancies via direct or indirect effects on the cellcycle machinery We investigated the role of p90RSK in lung adenocarcinomas and whether the inhibition of p90RSK diminishes cancer progression Moreover we investigated the involvement of glycogen synthase kinase3β GSK3β and osteopontin OPN in the p90RSKinduced lung adenocarcinoma progression p90RSK OPN and GSK3β protein expressions were examined in the A549 human lung adenocarcinoma cell line in the presence and absence of BID1870 BID a p90RSK inhibitor Gene expression of antiapoptotic and proapoptotic markers namely Bcl2 and Bax respectively were studied by reverse transcription polymerase chain reaction In addition the A549 lung adenocarcinoma cell line was characterized for cell proliferation using the MTT assay and cell migration using the scratch migration assay Our study revealed that total RSK1 protein expression is over expressed in the A549 human lung adenocarcinoma cell line an effect which is significantly reduced upon pretreatment with BID 6932 ± 1241  of control P  005 The inhibition of p90RSK also showed a significant suppression of cell proliferation 543 ± 673  of control P  001 and cell migration 18790 ± 1610  of control P  001 Treatment of the A549 cells with BID regressed the expression of Bcl2 mRNA 5692 ± 607  of control P  001 BID also regressed protein expression of OPN 7957 ± 532  of control P  005 and phosphoGSK3β 7304 ± 895  of control P  005 The p90RSK has an essential role in promoting tumor growth and proliferation in nonsmall cell lung cancer NSCLC BID may serve as an alternative cancer treatment in NSCLCNabeel Abdulrahman Noufira Poomakkoth Maiy Jaballa Sadaf Riaz Somaya Abdelaziz and Aya Issa performed the research Nabeel Abdulrahman Noufira Poomakkoth and Fatima Mraiche provided intellectual contributions and research assistance Nabeel Abdulrahman and Fatima Mraiche contributed in the analysis of the data Nabeel Abdulrahman Noufira Poomakkoth Maiy Jaballa and Fatima Mraiche wrote the paper

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