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Title of Journal: Mol Cell Biochem

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Abbravation: Molecular and Cellular Biochemistry

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Springer US

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DOI

10.1016/0002-9394(77)90184-2

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ISSN

1573-4919

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Silencing SATB1 inhibits proliferation of human os

Authors: Haiying Zhang Shanshan Qu Shuang Li Yang Wang Yulin Li Yimin Wang Zonggui Wang Ronggui Li
Publish Date: 2013/03/21
Volume: 378, Issue: 1-2, Pages: 39-45
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Abstract

It has been shown that overexpression of Special ATrich binding protein 1 SATB1 in breast cancer predicts a poor prognosis This study was aimed at investigating the effects of silencing SATB1 on mesenchymal derived human osteosarcoma U2OS cells and the underlying mechanisms The expressions of SATB1 and the related genes in the cells were detected by qRTPCR and/or Western Blotting SATB1 silencing was achieved by stable transfection with the vectors expressing small hairpin RNA versus SATB1 Cell proliferation was detected in a microplate reader with Cell Counting Kit8 and the cell cycle was analyzed by flow cytometry using a cell cycle detection kit The study found that SATB1 was particularly overexpressed in human osteosarcoma U2OS Silencing SATB1 inhibited the proliferation of U2OS It was found that inhibition of cell proliferation resulted from cell cycle arrest due to downregulated expression of CFGF and JunB The overexpression of SATB1 is responsible for abnormal proliferation of mesenchymal derived human Osteosatcoma U2OS cells indicating that silencing SATB1 expression in the cells might be developed as an efficient osteosarcoma therapy CTGF and JunB were involved in SATB1mediated proliferation of U2OS cellsThis study was supported in part by the Platform Fund for The Key Laboratory of Pathobiology Ministry of Education Jilin University No 450060481302 and Jilin University Initiation Fund No 450091105188 for “The Tang Aoqing Distinguished Professor” We would like to express our great appreciation to Dr Xuejin Su for his excellent technical assistance and to Professor F William Orr from the University of Manitoba in Canada for his great help in revising the manuscript

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