Authors: Tao Han Diancai Zhang Zan Fu Yueming Sun Wei Yang Cuimei Yuan
Publish Date: 2013/02/19
Volume: 377, Issue: 1-2, Pages: 219-227
Abstract
Retinolbinding protein 4 RBP4 is closely associated with a variety of abnormal glycolipid metabolism diseases such as insulin resistance obesity diabetes mellitus and metabolic syndrome Part 1 Twentyfour male C57/B6 mice were randomly divided into a normal control group group C n = 8 a gallstone group group G n = 8 and a medication group group M n = 8 Serum RBP4 levels were measured by ELISA RBP4 expression in liver and adipose tissue was detected by western blotting The transcription of RBP4 mRNA PPARγ mRNA in the liver was measured using realtime PCR The incidences of gallstone formation were 0/8 group C 7/8 group G and 2/8 group M p 001 Group G had significantly higher body weight adipose mass fasting glucose FG serum RBP4 total cholesterol TC triglycerides TG and highdensity lipoprotein cholesterol HDLC than group C In group M pioglitazone decreased body weight adipose mass levels of serum RBP4 FG TC and TG and it increased levels of HDLC Pioglitazone downregulated RBP4 expression in hepatic and adipose tissue and prevented decreases in PPARγmRNA levels induced by lithogenic diet Part 2 Metabolism indices including serum RBP4 FG TC TG HDLC lowdensity lipoprotein cholesterol LDLC alanine aminotransferase ALT and aspartate aminotransferase AST of 110 human cholesterol gallstone patients and 73 healthy controls were collected for further analysis Patients with gallstones had elevated levels of serum RBP4 FG TC TG ALT and AST and had decreased HDLC levels compared to those of healthy controls Elevated RBP4 is associated with the morbidity of cholesterol gallstones and metabolic syndrome RBP4 may play an important role in the course of cholesterol gallstone formation
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