Authors: Ulrike L MuellerLisse Oliver A Meissner Gregor Babaryka Margit Bauer Roger Eibel Christian G Stief Maximilian F Reiser Ullrich G MuellerLisse
Publish Date: 2006/03/28
Volume: 16, Issue: 10, Pages: 2259-2264
Abstract
Intraluminal optical coherence tomography OCT applies coherent light to provide crosssectional images with a spatial resolution of 10–25 μm We compared OCT and matching wholemount histology microscopy sections of porcine upper ureters ex vivo for visualization and delineation of different tissue layers of the ureteral wall Porcine ureters six specimens 24 quadrants were flushed with normal saline solution prior to insertion of the OCT catheter diameter 0014 inch OCT wavelength 1300±20 nm Crosssectional OCT images were obtained in marked locations before specimens were fixed in 4 formalin cut at marked locations wholemounted and stained with hematoxilin and eosin Visualization and delineation of different tissue layers of the ureteral wall by OCT was compared with matching histology by two independent observers O1O2 OCT distinguished tissue layers of the ureteral wall in all quadrants In OCT images O1/O2 delineated urothelium and lamina propria in 23/24 quadrants lamina propria and muscle layer in 19/16 quadrants inner and outer muscle layer in 13/0 quadrants and urothelial cell layers in 13/2 quadrants respectively Intraluminal OCT provides histologylike images of the ureter in porcine specimens ex vivo and reliably distinguishes between urothelium and deeper tissue layers of the ureteral wallParts of the work presented herein are based on results of doctoral thesis work in preparation by Margit Bauer at the Medical Faculty University of Munich Germany We gratefully acknowledge the technical assistance provided by staff members from LightLab Imaging Westford Mass USA Catheterbased intraluminal optical coherence tomography OCT of the ureter exvivo correlation with histology in porcine specimens
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