Protective Effects of Baicalin and Octreotide on M

Authors: Xi Ping Zhang Ling Zhang Ping Yang Rui Ping Zhang Qi Hui Cheng

Publish Date: 2007/06/05

Volume: 53, Issue: 2, Pages: 581-591

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Abstract

The improved Aho method was adopted to prepare SAP rat models via retrograde injection of 35 sodium taurocholate to the pancreatic duct The 135 SAP rat models after being prepared were randomly divided into the model group Baicalin treatment group and Octreotide treatment group with 45 rats in each group another 45 were selected to be the sham operation group which only received abdomen opening surgery The groups were then randomly divided into 3 h 6 h and 12 h groups with 15 rats in each group 10 min after successful modeling the Baicalin treatment group was first injected with a 5 Baicalin injection at a dose of 10 mg/100 g via external jugularvein passage followed by continuous intravenous administration 10 mg/h/100 g by microinfusion pump the Octreotide treatment group was first injected by Octreotide at a dose of 02 ug/100 g via external jugularvein passage followed by continuous intravenous transfusion 10 mg/h/100 g by microinfusion pump at a transfusion speed of 02 ug/h/100 g The sham operation group and model group were injected with saline of equivalent volume at the corresponding time points after operation The following observations were carried out 3 6 and 12 h after operation 1 mortalities of all rat groups followed by batch execution of rats and observation of the gross pathological changes of multiple organs 2 observation of the pathological changes of multiple organ samples fixed according to the relevant requirements after HE staining and 3 serum content of amylase NO malonaldehyde MDA and tumor necrosis factor alpha TNFα1 The survival rate of the sham operation group and all treatment groups was 100 whilst the 12 h survival of the model group was 6667 10/15 indicating a significant difference P  005 2 The gross pathological changes and changes under light microscopy of multiple organs aggravated with time after modeling The pathological changes of all treatment groups were milder than those of the model group at different time points by various degrees most obviously at 6 h and 12 h The gross pathological changes showed a similarity between the Octreotide and Baicalin treatment groups in terms of the pathological changes of pancreatic tissue The therapeutic effects of Octreotide on kidney and lung were superior to those in the Baicalin treatment group while the pathological manifestations of the Baicalin treatment group were superior to those of the Octreotide treatment group 3 There was no marked difference between the Baicalin and Octreotide treatment groups in terms of plasma amylase levels at all time points P  005 Although the plasma amylase levels of the Baicalin treatment group were lower than those of the model group at all time points the levels in the Baicalin treatment group were significantly lower than those in the model group only at 3 h P  005 and there was no marked difference in the levels between the Baicalin treatment group and model groups at 6 and 12 h P  005 the levels in the Octreotide treatment group were significantly lower than in the model group at 6 h P  005 and there was no marked difference between the levels in the Octreotide treatment group and model group at 3 h and 12 h P  005 4 The serum NO contents of the Baicalin treatment group were significantly lower than those of the model group P  005 while in the Octreotide treatment group it was obviously lower than in the model group at 3 and 12 h P  001 in this regard there was no marked difference between the Baicalin and Octreotide treatment groups at different time points P  005 5 The serum MDA contents of the Baicalin treatment group were significantly lower than those of the model group P  001 while in the Octreotide treatment group it was significantly less than the model group at 6 and 12 h P  005 and in the Baicalin treatment group was significantly less than in the Octreotide treatment group at 12 h P  005 6 There was no marked difference among the model group Baicalin treatment group and Octreotide treatment group in terms of serum TNFα content at 3 h and 12 h P  005 At 6 h the value in the Baicalin treatment group was significantly less than in the model group P  0001 in the Octreotide treatment group it was significantly less than in the model group P  0001 and the Octreotide treatment group it was significantly less than in the Baicalin treatment group P  001Both Baicalin and Octreotide have obvious protective effects on the multiple organ injury in SAP with mechanisms associated to manifold factors By comparing the pharmacologic effects of Octreotide and Baicalin we believe that Baicalin as a new drug has a protective effect on multiple organs of a SAP rat model similar to that of Octreotide and is worth further study and developmentThis work was supported by the technological foundation project of Traditional Chinese Medicine Science of Zhejiang province nos 2003C130 and 2004C142 the foundation project for medical science and technology of Zhejiang province no 2003B134 the grave foundation project for technological and development of Hangzhou No 2003123B19 intensive foundation project for technology of Hangzhou no 2004Z006 the foundation project for medical science and technology of Hangzhou no 2003A004 and the foundation project for technology of Hangzhou No 2005224 Note We claim that the article is original and will not have any financial interest in a company or its competitor and that all authors meet the criteria for authorship

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