Authors: Chee Y Ooi Tamara Pang Steven T Leach Tamarah Katz Andrew S Day Adam Jaffe
Publish Date: 2015/08/14
Volume: 60, Issue: 10, Pages: 2946-2952
Abstract
Defects in bacterial host defenses in the cystic fibrosis CF airways have been extensively investigated but the role of the intestinal innate immune system in CF is unknown Human βdefensin 2 HBD2 is an antimicrobial protein produced by epithelial surfaces and upregulated by inflammation Its expression in the CF intestine is unknownTo determine whether HBD2 was present in the feces of patients with CF and to compare fecal HBD2 levels between CF and healthy controls HC To compare fecal HBD2 levels in inflamed and noninflamed states as measured by fecal calprotectin as a secondary aimThirtythree CF patients and 33 HC were recruited All CF patients had detectable fecal HBD2 There was no difference between fecal HBD2 in CF and HC median IQR 491 197–772 versus 434 265–719 ng/g P = 07 Fecal calprotectin was significantly higher in the CF cohort than in HC median IQR 613 438–1438 versus 195 195–351 mg/kg P 00001 There was no difference in fecal HBD2 levels between CF subjects with fecal calprotectin ≥50 mg/kg and 50 mg/kg 505 196–802 versus 430 190–704 P = 07 There was no correlation between fecal HBD2 and calprotectin in CF r = 014 P = 04The authors are highly grateful to all the parents and children participating in the study We thank Dr John Morton Dr Yvonne Belessis Dr Penny Field Dr John Widger Rhonda Bell Rebecca McDonald Amanda Thomsen CF Clinic at Sydney Children’s Hospital Randwick Lily Nahidi Sydney Children’s Hospital Clinical Research Centre Dr Mark Jacobs Dr Kimberley Tan Dr Hughie Tsang Lourdes Lubrin Eye Clinic at Sydney Children’s Hospital Roxanne Strachan and Jung M Lee
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