Authors: Bu’Hussain Hayee Farooq Z Rahman Jane Tempero Sara McCartney Stuart L Bloom Anthony W Segal Andrew M Smith
Publish Date: 2010/10/09
Volume: 56, Issue: 5, Pages: 1482-1488
Abstract
Superoxide production in response to phorbolmyristyl acetate PMA was measured in neutrophils from 100 patients with CD compared to 50 healthy controls HCs and 50 patients with ulcerative colitis UC A further 22 CD and 10 HCs were studied using fMetLeuPhe fMLP and digestion of E coli by neutrophils was also evaluatedThe mean ± SEM PMAstimulated RB nmol O2/106 cells/min was 1086 ± 026 in HCs 976 ± 023 in CD P=002 and 1004 ± 028 in UC P=009 vs HC and 047 vs CD No significant effect of age gender or medication was observed The RB in three patients with presumed CD was found to be in the range expected in patients with inherited neutrophil disorders Stimulation with fMLP was calcium dependent and attenuated in patients on 5ASA Digestion of E coli by neutrophils was not different in HC vs CD 216 vs 2053 P=060The significant reduction in neutrophil RB in CD does not appear to result in defective bacterial digestion and is therefore unlikely play a major role in pathogenesis Three patients in this cohort of patients with presumed idiopathic CD were found to have a profound defect of the neutrophil RB A high index of suspicion for such patients is prudent as their prognosis can be improved by altering or augmenting the conventional treatment regimens employed for CDAll authors participated in the drafting and review of this manuscript Neutrophil respiratory burst assays were performed by BHH JT and FZR Digestion experiments were performed by BHH and AMS Patients were identified and characterized by BHH FZR SM and SLB This work was supported by a project grant from the CGD Trust BHH was awarded a project grant from the Broad Medical Research Program AWS was the recipient of a program grant from the Wellcome Trust
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