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Title of Journal: Arch Virol

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Abbravation: Archives of Virology

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Springer-Verlag

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DOI

10.1002/chin.199434293

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1432-8798

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Regulation of the Borna disease virus polymerase c

Authors: U Schneider M Naegele P Staeheli
Publish Date: 2004/04/22
Volume: 149, Issue: 7, Pages: 1409-1414
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Abstract

Borna disease virus BDV polymerase can be reconstituted by coexpression of the viral genes N P and L N codes for two proteins p39 and p38 resulting from alternative translation initiation Using a viral minireplicon system we observed that unlike p39 p38 was almost completely inactive when expressed with P and L alone Since BDV polymerase requires a 10–20 fold excess of N protein over P for high activity we determined whether p38 might serve as buffer that influences the viral NP ratio We demonstrate that p38 efficiently rescued BDV polymerase activity in cells expressing unfavorably high amounts of P

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