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Title of Journal: Arch Virol

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Abbravation: Archives of Virology

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Springer-Verlag

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DOI

10.1007/s00705-002-0810-x

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1432-8798

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Immunoelectron microscopy analysis of HCMV gpUL73

Authors: S Pignatelli P Dal Monte N Zini A Valmori N M Maraldi M P Landini
Publish Date: 2014/02/08
Volume: 147, Issue: 6, Pages: 1247-1256
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Abstract

 Human Cytomegalovirus HCMV UL73 encodes for a polymorphic structural glycoprotein gpUL73gN conserved among herpesviruses This study analyzed the intracellular and intraviral localization of gpUL73 by immunoelectronmicroscopy comparing the reactivity of two different antibodies We found that gN is an envelope component of the mature viral particle with at least a portion exposed at the virus surface and another at the internal side of the envelope Furthermore gpUL73 is also present in the matrix of dense bodies and “black holes” These results as well as immunoblotting analysis suggest that the two antibodies recognize different forms fully processed or unprocessed of gpUL73gN

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