Authors: Y Kono Y Yusnita A R Mohd Ali M Maizan S H Sharifah O Fauzia M Kubo A J Aziz
Publish Date: 2014/04/11
Volume: 147, Issue: 8, Pages: 1623-1630
Abstract
A virus named Oya virus was isolated in Vero cell cultures from the lungs of a pig suspected of Nipah virus infection The virus was revealed as a spherical enveloped RNA virus with a diameter of 79 nm For identification of Oya virus RTPCR was performed A common primer set for SRNA of the Simbu serogroup of the genus Bunyavirus was able to amplify a cDNA from Oya virus RNA The sequence data of the product revealed that the partial gene of Oya virus SRNA segment had 65–70 homology with published cDNA sequences of Simbu serogroup viruses The phylogenetic analysis of the data showed that the Oya virus is grouped in Simbu serogroup but is genetically distinct from the serogroup viruses that have been analyzed molecularly Serological surveys revealed that the virus distributed widely and densely in Malaysia
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