Authors: Jonathan E Breitenbach Holly J R Popham
Publish Date: 2013/02/27
Volume: 158, Issue: 7, Pages: 1517-1522
Abstract
A recent handful of studies have linked baculovirus infection with the induction of heat shock proteins a highly conserved family of cytoprotective proteins Here we demonstrate baculovirusstimulated upregulation of hsp70 transcription in the natural host Helicoverpa zea Larvae lethally infected with Helicoverpa zea single nucleopolyhedrovirus HzSNPV accumulated hsp70 transcripts throughout the 72hour course of infection in the midgut hemocytes and fat body While a maximal 17 or 15fold induction of hsp70 was noted in the midgut and hemocytes respectively by 72 hours postinfection the level of hsp70 transcription in the fat body of larvae was greater than two orders of magnitude higher than in mockinfected larvae These results were largely mirrored in cultures of infected cells and a potentiation effect was observed in cells that were both heat shocked and infected In contrast Spodoptera frugiperda multiple nucleopolyhedrovirus and ultravioletinactivated HzSNPV did not stimulate hsp70 transcription in these nonpermissive larvae and in cell culture respectively Taken together this report documents baculovirusmediated upregulation of hsp70 in the host and demonstrates the requirement for productive infection for hsp70 induction in vitro and in vivoThe US Department of Agriculture USDA prohibits discrimination in all its programs and activities on the basis of race color national origin age disability and where applicable sex marital status familial status parental status religion sexual orientation genetic information political beliefs reprisal or because all or part of an individual’s income is derived from any public assistance program Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture
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