Authors: ZhanYong Wei XueBin Wang XiaoDong Ning YaBin Wang HongYing Zhang DongFang Wang HongYing Chen BaoAn Cui
Publish Date: 2009/05/13
Volume: 154, Issue: 6, Pages: 999-1003
Abstract
This study investigated the inhibitory effect and mechanism of nitric oxide NO on porcine parvovirus PPV replication in PK15 cells The results showed that two NOgenerating compounds Snitrosolacetylpenicillamine SNAP and larginine LA at a noncytotoxic concentration could reduce PPV replication in a dosedependent manner and that this antiPPV effect could be reversed by the NO synthase NOS inhibitor Nnitrolarginine methyl ester lNAME By assaying the steps of the PPV life cycle we also show that NO inhibits viral DNA and protein synthesis This experiment provides a frame of reference for the study of the antiviral mechanism of NO
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